OrangerV1, Main, Exploration, bibRecord, 001C45

Utility of RAPD, ISSR, IRAP and REMAP markers for the genetic analysis of Citrus spp.

Identifieur interne : 001C45 ( Main/Exploration ); précédent : 001C44; suivant : 001C46

Utility of RAPD, ISSR, IRAP and REMAP markers for the genetic analysis of Citrus spp.

Auteurs : MANOSH KUMAR BISWAS [République populaire de Chine] ; QIANG XU [République populaire de Chine] ; Xiu-Xin Deng [République populaire de Chine]

Source :

Scientia horticulturae [ 0304-4238 ] ; 2010.

RBID : Pascal:10-0162602

Descripteurs français

Pascal (Inist)
- Marqueur RAPD, DNA microsatellite, Déterminisme génétique, Diversité génétique, Marqueur moléculaire, Polymorphisme, Ségrégation, Horticulture, Citrus, Marqueur IRAP.
Wicri :
- topic : Horticulture.

English descriptors

KwdEn :
- Citrus, Genetic determinism, Genetic diversity, Horticulture, Inter-retrotransposon amplified polymorphism marker, Microsatellite DNA, Molecular marker, Polymorphism, Random amplified polymorphic DNA marker, Segregation.

Abstract

The application and informativeness of RAPD, ISSR, IRAP and REMAP markers to study the genetic diversity and relationship among the Citrus and its relative genotypes were investigated. High levels of polymorphism were observed for all four marker systems. The RAPD technique generated the highest number of polymorphic bands and average number of polymorphic band per assay unit. Average limit of the discriminating power was very close to its actual discriminating power of each marker. The highest and the lowest values of effective number of patterns were obtained from the marker REMAP (5.94) and RAPD (4.48), respectively. Correlation between the genetic similarities matrices were estimated from all four markers using the Mantel matrix correspondence test, and results showed significant correlations among the RAPD, ISSR, IRAP and REMAP similarity matrices. The highest correlations were found comparing RAPD and ISSR markers, whereas RAPD and REMAP (r=0.143) markers were poorly correlated. To assess the usefulness of the overall information provided by these marker data for establishing phylogenetic relationships and Citrus germplasm classification, cluster analysis was performed. All four techniques, solely or in combination, discriminated the genotypes very efficiently and generated a high similarity in dendrogram topologies, although some differences were observed. The linkage analysis was conducted based on the segregation of 38 RAPD, 13 ISSR, 19 IRAP and 9 REMAP loci in 96 progeny of an intergeneric cross Citrus sinensis and Poncirus trifoliata. Among the 81 studied loci 65 loci distributed on five linkage groups. Comparing the result obtained with RAPD, ISSR, IRAP and REMAP markers in this study, IRAP and REMAP proved to be as a reliable molecular marker for fingerprinting, mapping and diversity study of Citrus and its relatives.

Affiliations:

République populaire de Chine

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to stream Main, to step Merge: 001C83
to stream Main, to step Curation: 001C45

Le document en format XML

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<term>Microsatellite DNA</term>
<term>Molecular marker</term>
<term>Polymorphism</term>
<term>Random amplified polymorphic DNA marker</term>
<term>Segregation</term>
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<term>DNA microsatellite</term>
<term>Déterminisme génétique</term>
<term>Diversité génétique</term>
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<front><div type="abstract" xml:lang="en">The application and informativeness of RAPD, ISSR, IRAP and REMAP markers to study the genetic diversity and relationship among the Citrus and its relative genotypes were investigated. High levels of polymorphism were observed for all four marker systems. The RAPD technique generated the highest number of polymorphic bands and average number of polymorphic band per assay unit. Average limit of the discriminating power was very close to its actual discriminating power of each marker. The highest and the lowest values of effective number of patterns were obtained from the marker REMAP (5.94) and RAPD (4.48), respectively. Correlation between the genetic similarities matrices were estimated from all four markers using the Mantel matrix correspondence test, and results showed significant correlations among the RAPD, ISSR, IRAP and REMAP similarity matrices. The highest correlations were found comparing RAPD and ISSR markers, whereas RAPD and REMAP (r=0.143) markers were poorly correlated. To assess the usefulness of the overall information provided by these marker data for establishing phylogenetic relationships and Citrus germplasm classification, cluster analysis was performed. All four techniques, solely or in combination, discriminated the genotypes very efficiently and generated a high similarity in dendrogram topologies, although some differences were observed. The linkage analysis was conducted based on the segregation of 38 RAPD, 13 ISSR, 19 IRAP and 9 REMAP loci in 96 progeny of an intergeneric cross Citrus sinensis and Poncirus trifoliata. Among the 81 studied loci 65 loci distributed on five linkage groups. Comparing the result obtained with RAPD, ISSR, IRAP and REMAP markers in this study, IRAP and REMAP proved to be as a reliable molecular marker for fingerprinting, mapping and diversity study of Citrus and its relatives.</div>
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Utility of RAPD, ISSR, IRAP and REMAP markers for the genetic analysis of Citrus spp.

Utility of RAPD, ISSR, IRAP and REMAP markers for the genetic analysis of Citrus spp.

Source :

Descripteurs français

English descriptors

Abstract

Links toward previous steps (curation, corpus...)

Le document en format XML

Pour manipuler ce document sous Unix (Dilib)

Pour mettre un lien sur cette page dans le réseau Wicri